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claudin 1 antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc claudin 1 antibodies
    Effect of Wogonoside on intestinal cell injury. A. Indirect immunofluorescence analysis demonstrates Wogonoside’s impact on ZO-1, Ocudin, <t>and</t> <t>Claudin-1</t> expression. B. Western Blot analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expressions. C. Wogonoside alleviated cell death. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.
    Claudin 1 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/claudin+1/Claudin-1+Antibody/pmc13590993-36-2-7
    Average 96 stars, based on 460 article reviews
    claudin 1 antibodies - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Wogonoside alleviates ulcerative colitis-induced senescence by activating AMPK in in vivo and in vitro models"

    Article Title: Wogonoside alleviates ulcerative colitis-induced senescence by activating AMPK in in vivo and in vitro models

    Journal: American Journal of Translational Research

    doi: 10.62347/NLXM4425

    Effect of Wogonoside on intestinal cell injury. A. Indirect immunofluorescence analysis demonstrates Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. B. Western Blot analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expressions. C. Wogonoside alleviated cell death. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.
    Figure Legend Snippet: Effect of Wogonoside on intestinal cell injury. A. Indirect immunofluorescence analysis demonstrates Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. B. Western Blot analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expressions. C. Wogonoside alleviated cell death. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.

    Techniques Used: Immunofluorescence, Analysis, Expressing, Western Blot

    Effect of Wogonoside on colonic tissue senescence in UC mice in vivo. A. Effect of Wogonoside on SA-β-gal levels. B. Wogonoside alleviated DSS-induced senescence, as assessed by p16/p21/p53 expression. C. Immunofluorescence analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. D. Wogonoside inhibited SASP levels. E. Effect of Wogonoside on the levels of ROS, MDA, GSH, and SOD. F. Wogonoside activated the AMPK signaling pathway. G. Effect of Wogonoside on the expression of OPA1, Mfn1, and Drp1. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.
    Figure Legend Snippet: Effect of Wogonoside on colonic tissue senescence in UC mice in vivo. A. Effect of Wogonoside on SA-β-gal levels. B. Wogonoside alleviated DSS-induced senescence, as assessed by p16/p21/p53 expression. C. Immunofluorescence analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. D. Wogonoside inhibited SASP levels. E. Effect of Wogonoside on the levels of ROS, MDA, GSH, and SOD. F. Wogonoside activated the AMPK signaling pathway. G. Effect of Wogonoside on the expression of OPA1, Mfn1, and Drp1. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.

    Techniques Used: Tissue, In Vivo, Expressing, Immunofluorescence, Analysis

    Related Articles

    Incubation:

    Article Title: Semaphorin 6D drives anti-tumor type I interferon responses to reprogram the tumor microenvironment in colorectal cancer.
    Article Snippet: Quantitative PCR (qPCR) assays were performed in triplicate on a CFX96 Real-Time System (Bio-Rad, Hercules, CA, USA) using SYBR Green Master Mix kit (Sigma-Aldrich, St. Louis, MO, USA), employing primer pairs listed in Table S2. .. Membranes were blocked with 5% milk for 2 hours subsequently incubated overnight at 4°C with specific primary antibodies, including: SEMA6D (sc393258) and GAPDH (sc-32233) from Santa Cruz Biotechnology (Dallas, TX, USA); E-cadherin (#3195), Caspase 3 (#3879), Cleaved Caspase 3 (#9585), Cleaved Caspase 8 (#9662), HSP90 (#9715),β-catenin (#8480), Claudin-1 (#13255), N-cadherin (#13116), vimentin (#5741), Plexin A4 (#3816), STAT1 (#14994), p-STAT1(#9167), IRF9 (#76684), STAT2 (#72604), p-STAT2 (#88410), HA tag (#3724) from Cell Signalling Technology (Massachusetts, USA); FLAG (F1804, Sigma-Aldrich); anti-Granzyme B (ab255598, Abcam, Cambridge, UK) and anti-Perforin (ab256453, Abcam, Cambridge, UK). ..

    Western Blot:

    Article Title: Metabolic Reprogramming and GM-CSF Secretion in Areca Nut-Activated Fibroblasts Drive Oral Precancer Progression.
    Article Snippet: The Human Phospho- kinase antibody array (ARY003B, R&D Systems) was employed to explore potential signaling pathways underlying the effect of ANE treated HGF conditioned medium on DOK cells, with the detailed procedure following the manufacturer's instructions. .. The primary antibodies used for Western blot included α- SMA (dilution 1:500, MA1- 06110, Invitrogen), FAP (dilution 1:500, ab28244, Abcam), Vimentin (dilution 1:5000, GTX100619, Genetex, Irvine, CA, USA), Claudin- 1 (dilution 1:1000, #4933, Cell Signaling Technology, Danvers, MA, USA), Zonula Occludens- 1 (ZO- 1) (dilution 1:1000, #5406, Cell Signaling Technology), p- EGFR Tyr1086 (dilution 1:1000, GTX133599, Genetex), and EGFR (dilution 1:1000, GTX100448, Genetex). .. The primary antibodies used for Western blot included α- SMA (dilution 1:500, MA1- 06110, Invitrogen), FAP (dilution 1:500, ab28244, Abcam), Vimentin (dilution 1:5000, GTX100619, Genetex, Irvine, CA, USA), Claudin- 1 (dilution 1:1000, #4933, Cell Signaling Technology, Danvers, MA, USA), Zonula Occludens- 1 (ZO- 1) (dilution 1:1000, #5406, Cell Signaling Technology), p- EGFR Tyr1086 (dilution 1:1000, GTX133599, Genetex), and EGFR (dilution 1:1000, GTX100448, Genetex).

    Recombinant:

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm 2 ) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin V-FITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino-2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green ® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23,227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13,663), Occludin (91,131), Claudin-1 (13,255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49,294) and Goat Anti-Rabbit IgG (H&L) HRP ( L35009 ) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411,308) was purchased from Ambion (Texas, USA). ..

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model.
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino AR TIC LE IN PR ES S acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm2) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin VFITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13663), Occludin (91131), Claudin-1 (13255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49294) and Goat AntiRabbit IgG (H&L) HRP (L35009) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411308) was purchased from Ambion (Texas, USA). ..

    Pore Size:

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm 2 ) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin V-FITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino-2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green ® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23,227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13,663), Occludin (91,131), Claudin-1 (13,255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49,294) and Goat Anti-Rabbit IgG (H&L) HRP ( L35009 ) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411,308) was purchased from Ambion (Texas, USA). ..

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model.
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino AR TIC LE IN PR ES S acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm2) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin VFITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13663), Occludin (91131), Claudin-1 (13255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49294) and Goat AntiRabbit IgG (H&L) HRP (L35009) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411308) was purchased from Ambion (Texas, USA). ..

    CCK-8 Assay:

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm 2 ) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin V-FITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino-2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green ® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23,227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13,663), Occludin (91,131), Claudin-1 (13,255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49,294) and Goat Anti-Rabbit IgG (H&L) HRP ( L35009 ) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411,308) was purchased from Ambion (Texas, USA). ..

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model.
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino AR TIC LE IN PR ES S acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm2) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin VFITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13663), Occludin (91131), Claudin-1 (13255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49294) and Goat AntiRabbit IgG (H&L) HRP (L35009) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411308) was purchased from Ambion (Texas, USA). ..

    RNA Extraction:

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm 2 ) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin V-FITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino-2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green ® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23,227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13,663), Occludin (91,131), Claudin-1 (13,255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49,294) and Goat Anti-Rabbit IgG (H&L) HRP ( L35009 ) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411,308) was purchased from Ambion (Texas, USA). ..

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model.
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino AR TIC LE IN PR ES S acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm2) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin VFITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13663), Occludin (91131), Claudin-1 (13255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49294) and Goat AntiRabbit IgG (H&L) HRP (L35009) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411308) was purchased from Ambion (Texas, USA). ..

    Reverse Transcription:

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm 2 ) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin V-FITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino-2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green ® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23,227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13,663), Occludin (91,131), Claudin-1 (13,255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49,294) and Goat Anti-Rabbit IgG (H&L) HRP ( L35009 ) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411,308) was purchased from Ambion (Texas, USA). ..

    Article Title: Quercetin ameliorates HIV-1 gp120 protein-induced intestinal barrier dysfunction by inhibiting the activation of the ERK1/2 signaling pathway in a Caco-2 cell model.
    Article Snippet: MEM cell culture medium, sodium pyruvate, non-essential amino AR TIC LE IN PR ES S acids (NEAA) and double antibody were purchased from Prosperity Life Science and Technology Co. Ltd. (Wuhan, China). .. Fetal bovine serum (F8318) and lipopolysaccharide LPS (L4391), fluorescein isothiocyanate-labeled dextran (FITC-dextran, FD4) and Bovine Serum Albumin (A1933) were purchased from Sigma-Aldrich (St. Louis, MO, USA), recombinant human HIV-1 gp120 (CN54) protein (ab174070) and secondary anti-goat anti-rabbit IgG Alexa Fluor® 488 (ab150077) were purchased from Abcam (Cambridge, UK), Quercetin (DR10620) was purchased from Dingrui Chemical Co Ltd (Shanghai, China), 12-well transwell culture chambers (3401) (pore size 0.4 μm, diameter 12 mm, growth area 1.12 cm2) were purchased from Corning (Cambridge, MA, USA), alkaline phosphatase kits (A059) were purchased from Nanjing Jianjian Bioengineering Institute (Nanjing, China), CCK-8 kits (C0043), Annexin VFITC apoptosis detection kits (C1062), anti-fluorescence quencher (P0126), and 4′,6-diamidino2-phenylindole (DAPI) (C1005) were purchased from Biyuntian Biotechnology (Shanghai, China), Total RNA Extraction Kit (9767), cDNA Reverse Transcription Kit (RR047A), and TB Green® Premix Ex TaqTM II (RR820A) were purchased from Takara Bio (Shiga, Japan), and BCA Protein Analysis Kits (23227) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada), U0126 (9903), primary antibody ZO-1 (13663), Occludin (91131), Claudin-1 (13255), phospho-p44/42 ERK1/2 ( 4370), p44/42 ERK1/2 (4695), phospho-MLC (3671), and MLC (8505) were purchased from Cell Signaling Technology (Danvers, MA, USA), MLCK (YA3133) was purchased from Med Chem Express (New Jersey, USA), β-actin (49294) and Goat AntiRabbit IgG (H&L) HRP (L35009) were purchased from Signalway Antibody (College Park, MD, USA), and TRIzol (411308) was purchased from Ambion (Texas, USA). ..

    Control:

    Article Title: Orally deliverable Perilla frutescens-derived nanovesicles as natural bioactive nanocarriers for colon-targeted colitis therapy via microenvironment reprogramming
    Article Snippet: Effective oral therapy for inflammatory bowel disease (IBD) requires overcoming gastrointestinal barriers to modulate the dysregulated mucosal niche.. Here, we present edible nanovesicles derived from Perilla frutescens (PLENs) as an intrinsically stable, bioactive nanotherapeutic.. Multi-omics profiling defined a robust lipid-bilayer architecture encapsulating a synergistic cargo of proteins, miRNAs, and antioxidant metabolites.

    other:


    Article Title: Semaphorin 6D drives anti-tumor type I interferon responses to reprogram the tumor microenvironment in colorectal cancer
    Article Snippet: Quantitative PCR (qPCR) assays were performed in triplicate on a CFX96 Real-Time System (Bio-Rad, Hercules, CA, USA) using SYBR Green Master Mix kit (Sigma-Aldrich, St. Louis, MO, USA), employing primer pairs listed in Table .



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    Effect of Wogonoside on intestinal cell injury. A. Indirect immunofluorescence analysis demonstrates Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. B. Western Blot analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expressions. C. Wogonoside alleviated cell death. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.

    Journal: American Journal of Translational Research

    Article Title: Wogonoside alleviates ulcerative colitis-induced senescence by activating AMPK in in vivo and in vitro models

    doi: 10.62347/NLXM4425

    Figure Lengend Snippet: Effect of Wogonoside on intestinal cell injury. A. Indirect immunofluorescence analysis demonstrates Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. B. Western Blot analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expressions. C. Wogonoside alleviated cell death. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.

    Article Snippet: ZO-1 and Claudin-1 antibodies were obtained from CST, USA.

    Techniques: Immunofluorescence, Analysis, Expressing, Western Blot

    Effect of Wogonoside on colonic tissue senescence in UC mice in vivo. A. Effect of Wogonoside on SA-β-gal levels. B. Wogonoside alleviated DSS-induced senescence, as assessed by p16/p21/p53 expression. C. Immunofluorescence analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. D. Wogonoside inhibited SASP levels. E. Effect of Wogonoside on the levels of ROS, MDA, GSH, and SOD. F. Wogonoside activated the AMPK signaling pathway. G. Effect of Wogonoside on the expression of OPA1, Mfn1, and Drp1. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.

    Journal: American Journal of Translational Research

    Article Title: Wogonoside alleviates ulcerative colitis-induced senescence by activating AMPK in in vivo and in vitro models

    doi: 10.62347/NLXM4425

    Figure Lengend Snippet: Effect of Wogonoside on colonic tissue senescence in UC mice in vivo. A. Effect of Wogonoside on SA-β-gal levels. B. Wogonoside alleviated DSS-induced senescence, as assessed by p16/p21/p53 expression. C. Immunofluorescence analysis examining Wogonoside’s impact on ZO-1, Ocudin, and Claudin-1 expression. D. Wogonoside inhibited SASP levels. E. Effect of Wogonoside on the levels of ROS, MDA, GSH, and SOD. F. Wogonoside activated the AMPK signaling pathway. G. Effect of Wogonoside on the expression of OPA1, Mfn1, and Drp1. ns = not significant; *P<0.05, **P<0.01, ***P<0.001.

    Article Snippet: ZO-1 and Claudin-1 antibodies were obtained from CST, USA.

    Techniques: Tissue, In Vivo, Expressing, Immunofluorescence, Analysis

    Oral SH restored the BBB and intestinal barrier impairment in the MPTP-induced PD mouse models. ( A ) Representative captures of immunofluorescence in the colon of ZO-1. ( B ) Representative WB bands of ZO-1, Occludin, and Claudin-1 in the colon. ( C ) Representative WB bands of ZO-1, Occludin, and Claudin-5 in the midbrain containing the SN. ( D ) ZO-1 integrity score in the colon. ( E ) The density analysis of ZO-1, Occludin, and Claudin-1 WB in the colon. ( F ) The density analysis of ZO-1, Occludin, and Claudin-5 WBs in the midbrain containing the SN. (In this figure, n = 3 for each group. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 versus the MPTP group.).

    Journal: International Journal of Molecular Sciences

    Article Title: Oral Sodium Hyaluronate Reshapes Gut Microbiota Composition and Suppresses the LPS-TLR4/NF-κB Pathway to Exert Neuroprotection in MPTP-Induced Parkinson’s Disease

    doi: 10.3390/ijms27156573

    Figure Lengend Snippet: Oral SH restored the BBB and intestinal barrier impairment in the MPTP-induced PD mouse models. ( A ) Representative captures of immunofluorescence in the colon of ZO-1. ( B ) Representative WB bands of ZO-1, Occludin, and Claudin-1 in the colon. ( C ) Representative WB bands of ZO-1, Occludin, and Claudin-5 in the midbrain containing the SN. ( D ) ZO-1 integrity score in the colon. ( E ) The density analysis of ZO-1, Occludin, and Claudin-1 WB in the colon. ( F ) The density analysis of ZO-1, Occludin, and Claudin-5 WBs in the midbrain containing the SN. (In this figure, n = 3 for each group. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 versus the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 versus the MPTP group.).

    Article Snippet: Membranes were incubated at 4 °C overnight with the following primary antibodies: rabbit anti-β-actin antibody (1:50,000, AC026, Abclonal, Wuhan, China), mouse anti-TH antibody (1:1000, A0028, Abclonal, Wuhan, China), rabbit anti-TNF-α antibody (1:1000, A20851, Abclonal, Wuhan, China), rabbit anti-IL-1β antibody (1:1000, A11369, Abclonal, Wuhan, China), rabbit anti-IL-6 antibody (1:1000, A0286, Abclonal, Wuhan, China), goat anti-COX-2 antibody (1:1000, A1253, Abclonal, Wuhan, China), mouse anti-TLR4 antibody (1:1000, A5258, Abclonal, Wuhan, China), rabbit anti-MyD88 antibody (1:400, A0980, Abclonal, Wuhan, China), rabbit anti-IκB-α antibody (1:1000, A24742, Abclonal, Wuhan, China), rabbit anti-ZO-1 antibody (1:1000, A0659, Abclonal, Wuhan, China), mouse anti-claudin-1 antibody (1:1000, Abclonal, Wuhan, China), rabbit anti-occludin antibody (1:1000, Abclonal, China), and mouse anti-claudin-5 antibody (1:1000, A10207 , Abclonal, Wuhan, China).

    Techniques: Immunofluorescence, Control