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Expression of PAR2, IL-17, and TJ proteins in Malassezia folliculitis (A and B) PAR2, IL-17, ZO-1, occludin, <t>and</t> <t>claudin-1</t> were validated in MF and CTRL skin tissue by immunohistochemical staining (IHC). Values are mean ± SD of six samples from two independent experiments. Statistical analysis was performed using a two-way ANOVA with Šídák’s multiple comparisons test (∗ p < 0.05; ∗∗∗ p < 0.001). (C and D) The spores rate of CTRL and MF groups by Periodic Acid-Schif staining. Purple arrows indicate the spores. Scale bars, 50 μm. Data are presented as mean ± SD of triplicate wells from three independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t tests (∗∗ p < 0.01).
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Expression of PAR2, IL-17, and TJ proteins in Malassezia folliculitis (A and B) PAR2, IL-17, ZO-1, occludin, and claudin-1 were validated in MF and CTRL skin tissue by immunohistochemical staining (IHC). Values are mean ± SD of six samples from two independent experiments. Statistical analysis was performed using a two-way ANOVA with Šídák’s multiple comparisons test (∗ p < 0.05; ∗∗∗ p < 0.001). (C and D) The spores rate of CTRL and MF groups by Periodic Acid-Schif staining. Purple arrows indicate the spores. Scale bars, 50 μm. Data are presented as mean ± SD of triplicate wells from three independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t tests (∗∗ p < 0.01).

Journal: iScience

Article Title: PAR2-β-arrestin 2-ERK axis mediates Malassezia globosa -induced IL-17 response by disrupting ZO-1 in keratinocytes

doi: 10.1016/j.isci.2026.115646

Figure Lengend Snippet: Expression of PAR2, IL-17, and TJ proteins in Malassezia folliculitis (A and B) PAR2, IL-17, ZO-1, occludin, and claudin-1 were validated in MF and CTRL skin tissue by immunohistochemical staining (IHC). Values are mean ± SD of six samples from two independent experiments. Statistical analysis was performed using a two-way ANOVA with Šídák’s multiple comparisons test (∗ p < 0.05; ∗∗∗ p < 0.001). (C and D) The spores rate of CTRL and MF groups by Periodic Acid-Schif staining. Purple arrows indicate the spores. Scale bars, 50 μm. Data are presented as mean ± SD of triplicate wells from three independent experiments. Statistical significance was determined using unpaired two-tailed Student’s t tests (∗∗ p < 0.01).

Article Snippet: Primer of Claudin-1 (Forward, 5′-AGGTACGAATTTGGTCAGG CTCTC-3’; Reverse, 5′-GGGAC AGGAACAGCAAAGTAGGG-3′) , Sangon Biotech , N/A.

Techniques: Expressing, Immunohistochemical staining, Staining, Two Tailed Test